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How can GIA scientists know if a natural sapphire has received heat treatment? One clue is through its inclusions. Sapphires that formed in metamorphic rock often contain carbon dioxide fluid inclusions or negative crystals. If heat-treated, the carbon dioxide escapes from these inclusions so that they become empty. When the stone and its inclusions are cooled to below 31.5°C, a visible carbon dioxide vapor bubble forms within the liquid. This vapor bubble and the presence of liquid indicate tha
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