Use an extraction buffer to isolate nuclei from tissue. Image Credit: DeNovix Inc. 10X Genomicsâ„¢ and Miltenyi Biotecâ„¢ are registered trademarks. Adjust AO/PI to room temperature. Add the AO/PI ...
Isolating nuclei is essential for single nuclear workflows like ATAC-sequencing and RNA-sequencing. Obtaining non-clustered, debris-free samples is important for successful library preparation. Even ...