Problem: Transfecting any siRNA may lead to off-target changes in gene expression. Controls: Do at least one negative control that doesn't target any endogenous gene, says Ruiz-Vela. He generally uses ...

In scientific research, controls are employed as standards. By separating the control from the samples exposed to an experimental condition, such as the introduction of an analyte, the effects of the ...

Though an essential component of experimental design, negative controls are not implemented widely by antibody manufacturers and distributors. Neglecting this important validation step has resulted in ...